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web-based shrna design tool  (GenScript corporation)

 
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    Structured Review

    GenScript corporation web-based shrna design tool
    <t>miR-shRNA</t> based gene silence <t>of</t> <t>tppp3</t> diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.
    Web Based Shrna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/web-based+shrna+design+tool/shrna+target+finder/pmc05702462-139-4-8
    Average 90 stars, based on 1 article reviews
    web-based shrna design tool - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo"

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2017.00375

    miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.
    Figure Legend Snippet: miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.

    Techniques Used: shRNA, Sequencing, Quantitative RT-PCR, Expressing, Imaging, Two Tailed Test

    Related Articles

    shRNA:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Sequencing:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Quantitative RT-PCR:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Expressing:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Imaging:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Two Tailed Test:

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).



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    <t>miR-shRNA</t> based gene silence <t>of</t> <t>tppp3</t> diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.
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    Image Search Results


    miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo

    doi: 10.3389/fnmol.2017.00375

    Figure Lengend Snippet: miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.

    Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

    Techniques: shRNA, Sequencing, Quantitative RT-PCR, Expressing, Imaging, Two Tailed Test